Part:BBa_K2423001:Design

CsADH-2946 under the control of a constitutive promoter (BBa_J23106)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 195
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 602
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1119
  Illegal SapI.rc site found at 227
  

Design Notes

We made a complete device including promoter and RBS. We also included a 6x his tag for easy purification of the expressed protein.

Source

Crocus sativus